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Image Search Results
Journal: PloS one
Article Title: Assessing the role of STAT3 in DC differentiation and autologous DC immunotherapy in mouse models of GBM.
doi: 10.1371/journal.pone.0096318
Figure Lengend Snippet: Figure 2. The role of STAT3 signaling in the differentiation and expansion of DCs by Flt3L and GM-CSF. A. WT and STAT3 null bone marrow cells were cultured in the presence of hFlt3L (100 ng/ml) for 8 days then subsequently analyzed by flow cytometry for DC subtypes. Expression of CD45R was used to distinguish pDCs (CD11c+/CD45R+) from cDCs (CD11c+/CD45R2). The total number of CD11c+ DCs expanded from WT and STAT3 KO bone marrow was quantified from multiple independent bone marrow cultures (*, p,0.05; two-tail students t-test). B. Flow cytometry and quantification of GM-CSF-derived (40 ng/ml) BMDCs from WT and STAT3 null bone marrow cells. C. WT and STAT3 deficient mice were
Article Snippet: Recombinant mouse GM-CSF was procured from Abd Serotec (Raleigh, NC; cat# PMP82) and
Techniques: Cell Culture, Flow Cytometry, Expressing, Derivative Assay